GSM5175649: S11 HelaCAS9 HIF1A Hx rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
HIF1A
Cell type
Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Hela cells_control_CRISPR
cell line
HeLa
genotype
Wildtype
ambient o2
1%
treatment time
6 hours
chip antibody
HIF1A
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody for HIF-1alpha, HIF-2alpha or HIF-1beta. Libraries were constructed according to Illumina protocols Each sample was sequenced over two lanes, using 150bp paired-end reads