Antigen

Antigen Class

TFs and others

Antigen

HIF1A

Cell type

Cell type Class

Uterus

Cell type

HeLa

Primary Tissue

Cervix

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

Hela cells_control_CRISPR

cell line

HeLa

genotype

Wildtype

ambient o2

1%

treatment time

6 hours

chip antibody

HIF1A

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody for HIF-1alpha, HIF-2alpha or HIF-1beta. Libraries were constructed according to Illumina protocols Each sample was sequenced over two lanes, using 150bp paired-end reads

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

30517669

Reads aligned (%)

94.4

Duplicates removed (%)

19.1

Number of peaks

1660 (qval < 1E-05)

hg19

Number of total reads

30517669

Reads aligned (%)

93.7

Duplicates removed (%)

19.4

Number of peaks

1032 (qval < 1E-05)