Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HIF1A

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
Hela cells_control_CRISPR
cell line
HeLa
genotype
Wildtype
ambient o2
1%
treatment time
6 hours
chip antibody
HIF1A

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody for HIF-1alpha, HIF-2alpha or HIF-1beta. Libraries were constructed according to Illumina protocols Each sample was sequenced over two lanes, using 150bp paired-end reads

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
30517669
Reads aligned (%)
94.4
Duplicates removed (%)
19.1
Number of peaks
1660 (qval < 1E-05)

hg19

Number of total reads
30517669
Reads aligned (%)
93.7
Duplicates removed (%)
19.4
Number of peaks
1032 (qval < 1E-05)

Base call quality data from DBCLS SRA